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Título del Articulo: Differential population history in the migratory catfishes Brachyplatystoma flavicans and Pseudoplatystoma fasciatum (Pimelodidae) from the Bolivian Amazon assessed with nuclear and mitochondrial DNA markers

Autores UMSS: Coronel J.S., Van Damme P.A.

Autores: Maes G.E., Claus S., Volckaert F.A.M.

Facultad: Ciencias y Tecnología

Centro: Unidad de Limnología y Recursos Acuáticos (ULRA)

Editorial: Blackwell, Oxford

Fecha de Publicación: 2004     Numero de Paginas 859-868

Abstract:
The catfishes Brachyplatystoma flavicans(n = 49) and Pseudoplatystoma fasciatum(n = 69) showed comparable low allozyme diversities (He = 0·012 and 0·009–0·028, respectively), but contrasting PCR‐RFLP restriction site mitochondrial DNA diversities (three haplotypes: π = 0·034–0·092 and five haplotypes: π = 0·001–0·023, respectively) in the Rio Ichilo and Beni (Bolivia). Genetic homogeneity between samples was high for B. flavicans and lower for P. fasciatum. Based on mitochondrial diversity, both species probably experienced a historic population reduction but at different time scales.

Para mas informacion aproximarse por : Unidad de Limnología y Recursos Acuáticos (ULRA) en la facultade de Ciencias y Tecnología


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Título del Articulo: Temporary shallow pools in high-Andes 'bofedal' peatlands: A limnological characterization at different spatial scales

Autores UMSS: Coronel J.S., Maldonado M.

Autores: Declerck S., Ollevier F., Brendonck L.

Facultad: Ciencias y Tecniología

Centro: Unidad de Limnología y Recursos Acuáticos (ULRA)

Editorial: Soc phys hist naturelle geneve

Abstract:
This study is the first to characterize the limnological features of high-altitude peatland pools in the Andes cordillera (>4000m). Data were collected at two different spatial scales: the scale of individual patches of peatland (bofedales; areas rangingbetween 0.01 and 10 ha) and the scale of ‘localities’ (areas of approximately 7 km2). Patterns of variable associations weresimilar at the different spatial scales. The major gradient of variation appeared to represent a productivity gradient. Waterplant cover and richness were associated with alkalinity

Para mas informacion aproximarse por : Unidad de Limnología y Recursos Acuáticos (ULRA) en la facultade de Ciencias y Tecniología


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Título del Articulo: Essential oils from bolivia. VII. Myrtaceae: Myrcianthes osteomeloides (Rusby) McVaugh and Myrcianthes pseudomato (Legrand) McVaugh

Autores UMSS: Arze J.B.L.

Autores: Jean F.I., Gagnon H., Collin G., Garneau F.X., Pichette A.

Facultad: Ciencias y Tecnología

Centro: Centro de Tecnología Agroindustrial (CTA)

Fecha de Publicación: 2005     Numero de Paginas 64-65

Abstract:
The chemical composition of the essential oils of Myrcianthes osteomeloides and M. pseudomato was studied by GC and GC/MS. Myrcianthes osteomeloides oil contained 1,8-cineole (55.7%) as the major component followed by alpha-pinene (17.9%), alpha-terpineol (8.5%) and beta-pinene (4.6%). We identified 1,8-cineole (24.4%), alpha-pinene (17.1%), linalool (11.7%), limonene (8.5%) and gamma-terpinene (7.3%) as main constituents in M. pseudomato oil.

Para mas informacion aproximarse por : Centro de Tecnología Agroindustrial (CTA) en la facultade de Ciencias y Tecnología


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Título del Articulo: IgM-antibody responses of chickens to salivary antigens of Triatoma infestans as early biomarkers for low-level infestation of triatomines

Autores UMSS: Medrano-Mercado N.,

Autores: Schwarz A.,Billingsley P.F., Schaub G.A., Sternberg J.M.

Facultad: Ciencias y Tecnología

Centro: Laboratorio de Inmunoparasitología y Chagas (ICIP-BIO) - Departamento de Biología

Editorial: Elsevier, Kidlington

Fecha de Publicación: 2010     Numero de Paginas 1295-1302

Abstract:
The recombinant form of a highly immunogenic 14.6 kDa protein in Triatoma infestans saliva (rTiSP14.6) is a potential epidemiological marker for the detection of triatomine bug populations using IgG responses in peridomestic chickens. However, the persistence of the IgG response prevents it being of value for several months in areas where triatomine control programmes have been implemented. In this investigation, IgM-antibody reactions to crude salivary antigens or rTiSP14.6 decayed rapidly after exposure of chickens and were measurable for only 18 days after a single challenge with T. infestans. In serial exposure experiments, chickens from low and high exposure groups showed no significant differences in anti-saliva and anti-rTiSP14.6 IgM-antibody titres. Highly immunogenic salivary antigens of 12 and 14 kDa were recognised by all chicken sera. Sera from peridomestic chickens from sites of known T. infestans infestation in Bolivia also recognised these two antigens and no differences in the IgM responses of sera from chickens from low and high infestation households were detected. IgM responses were specific to infested households and could not be detected in sera from non-infested households. Cross-reactivity studies showed that at least four other triatomine species share the 14.6 kDa salivary antigen. No IgM responses were detected against salivary proteins of mosquitoes and sandflies. Thus, we believe that rTiSP14.6 represents a promising epidemiological marker for the detection of low numbers of triatomines in peridomestic habitats, and the comparison of IgM and IgG responses can be used to detect re-infestation soon after insecticide-based control programmes.

Sitio web: sciencedirect

Para mas informacion aproximarse por : Laboratorio de Inmunoparasitología y Chagas (ICIP-BIO) - Departamento de Biología en la facultade de Ciencias y Tecnología


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Título del Articulo: Development and evaluation of methods for starch dissolution using asymmetrical flow field-flow fractionation. Part I: Dissolution of amylopectin

Autores UMSS: Perez-Rea D.

Autores: Bergenståhl B., Nilsson L.

Facultad: Ciencias y Tecnología

Centro: Centro de Alimentos y Productos Naturales (CAPN)

Editorial: Springer Berlin Heidelberg

Fecha de Publicación: 2016     Numero de Paginas 1531-1532

Abstract:
We have investigated methods of starch dissolution with the aim of finding an optimum method to completely dissolve starch granules to form a molecularly dissolved starch solution without degradation of the polymers. Glycogen was used as a model molecule for amylopectin, to identify the dissolution conditions under which the degradation of the polymers was limited or not present. Dissolution was performed in water with temperatures up to 200 °C, facilitated by the use of heating in an autoclave or a microwave oven, or in dimethyl sulfoxide (DMSO) at 100 °C. Waxy maize starch was chosen due to its high content of amylopectin and very low content of amylose. The degree of starch dissolution under different conditions was determined enzymatically. The effect of different dissolution conditions on the molar mass and root-mean-square radius of the polymers was determined with asymmetrical flow field-flow fractionation coupled to multi-angle light scattering and differential refractive index (AF4-MALS-dRI) detectors under aqueous conditions. The results suggest that reliable and accurate size separation and characterization of amylopectin can be obtained by dissolution of starch granules in an aqueous environment at 140 °C by autoclaving or in DMSO at 100 °C. The results also clearly show an upper limit for heat treatment of starch, above which degradation cannot be avoided.

Para mas informacion aproximarse por : Centro de Alimentos y Productos Naturales (CAPN) en la facultade de Ciencias y Tecnología


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Título del Articulo: Trans-Amazonian natal homing in giant catfish

Autores UMSS: Carvajal-Vallejos F.

Autores: Duponchelle F., Pouilly M., Pécheyran C., Hauser M., Renno J.-F., Panfili J., Darnaude A.M., García-Vasquez A., García-Dávila C., Doria C., Bérail S., Donard A., Sondag F., Santos R.V., Nuñez J., Point D., Labonne M., Baras E.

Facultad: Ciencias y Tecnología

Centro: Unidad de Limnología y Recursos Acuáticos (ULRA)

Fecha de Publicación: 2016     Numero de Paginas 1511-1520

Abstract:
Knowledge of fish migration is a prerequisite to sustainable fisheries management and preservation, especially in large international river basins. In particular, understanding whether a migratory lifestyle is compulsory or facultative, and whether adults home to their natal geographic area is paramount to fully appraise disruptions of longitudinal connectivity resulting from damming. In the Amazon, the large migratory catfishes of the Brachyplatystoma genus are apex predators of considerable interest for fisheries. They are believed to use the entire length of the basin to perform their life cycle, with hypothesized homing behaviours. Here, we tested these hypotheses, using the emblematic B. rousseauxii as a model species. We sampled adults close to major breeding areas in the Amazon basin (upper Madeira and upper Amazonas) and assessed their lifetime movements by measuring variations in 87Sr/86Sr along transverse sections of their otoliths (ear stones) using laser ablation multicollector mass spectrometry (LA-MC-ICPMS). We demonstrate that larvae migrate downstream from the Andean piedmont to the lower Amazon, where they grow over a protracted period before migrating upstream as adults.

Para mas informacion aproximarse por : Unidad de Limnología y Recursos Acuáticos (ULRA) en la facultade de Ciencias y Tecnología


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Título del Articulo: Composition of the essential oil of Porophyllum ruderale (Jacq.) Cass. from Bolivia

Autores UMSS: Loayza I., De Groot W.

Autores: Lorenzo D., Dellacassa E., Mondello L., Dugo G.

Facultad: Ciencias y Tecnología

Centro: Centro de Tecnología Agroindustrial (CTA) - Programa Agroquímico

Editorial: Wiley, Brisbane / Wiley, Chichester / Wiley, New York, NY

Fecha de Publicación: 1999     Numero de Paginas 393-398

Abstract:
The essential oil of aerial parts of Porophyllum ruderale (Jacq.) Cass. was analysed by HRGC, HRGC–MS (quadrupole) and 26 components (93% of the total composition) were identified. The enantiomeric distribution of β‐pinene, sabinene, 4‐terpineol and α‐terpineol was studied by multidimensional HRGC–HRGC. The major component was sabinene (64%) with an enantiomeric purity of 97% for ( )‐sabinene. Copyright © 1999 John Wiley & Sons, Ltd.

Para mas informacion aproximarse por : Centro de Tecnología Agroindustrial (CTA) - Programa Agroquímico en la facultade de Ciencias y Tecnología


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Título del Articulo: Nutrient limitation of bacteria and sources of nutrients supporting nutrient-limited bacterial growth in an Amazonian floodplain lake

Autores UMSS: Rejas D.

Autores: Muylaert K., De Meester L.

Facultad: Ciencias yTecnología

Centro: Unidad de Limnología y Recursos Acuáticos (ULRA)

Editorial: Inter-Research, Oldendorf/Luhe

Fecha de Publicación: 2005     Numero de Paginas 57-67

Abstract:
ABSTRACT: Laguna Bufeos is a white-water floodplain lake located in the upper Amazon basin (Ichilo River, Bolivia). To study nutrient limitation of bacteria and to determine the sources supporting nutrient-limited bacterial growth, 5 nutrient deletion/dilution experiments were carried out in Laguna Bufeos. We carried out 3 experiments during 3 consecutive low-water seasons and 2 experiments during 1 high-water season. No evidence of N or P limitation was observed during the 2 experiments in the high-water season. Conversely, bacteria were limited by P in the 3 experiments in the low-water season. Limitation by N occurred only in one of the low-water experiments. Nutrient-limited bacterial growth rates equaled 37 to 86% of nutrient-saturated growth rates. Nutrients recycled by microzooplankton, mainly heterotrophic nanoflagellates and oligotrich ciliates, were the major nutrient source supporting P- or N-limited growth. Our results suggest a strong control of bacterial growth rates by P in Amazonian white-water lakes during the low-water season, while this limitation is alleviated during the high-water season, probably through supply of new nutrients from the river. The strong nutrient limitation of bacteria and the dependence of bacteria on nutrients supplied by their predators can be expected to slow down the decomposition of organic matter in Amazonian floodplain lakes. This may partly explain why these ecosystems are often rich in organic matter.

Para mas informacion aproximarse por : Unidad de Limnología y Recursos Acuáticos (ULRA) en la facultade de Ciencias yTecnología


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Título del Articulo: High-altitude peatland temporary pools in Bolivia house a high cladoceran diversity

Autores UMSS: Coronel J.S.

Autores: Declerck S., Brendonck L.

Facultad: Ciencias y Tecnología

Centro: Unidad de Limnología y Recursos Acuáticos (ULRA)

Editorial: Society of Wetlands Scientists

Fecha de Publicación: 2007     Numero de Paginas 1166 - 1174

Abstract:
We studied richness and composition of the cladoceran species in 61 temporary peat-pools (pools within high-altitude peatland mires) in the Cordillera del Tunari in Cochabamba (Bolivia) during one wet season. Of the 21 species collected, two were new to science and five were new records to Bolivia. Across all 61 pools, species richness per pool varied from 3–16 (mean  =  8.3 2.8 SD). Rarefaction analysis revealed that more than 80% of all collected species was represented by a subsample of 25 pools. This is the first study to comprehensively present the cladoceran fauna of temporary peat-pools in the high Andes. The cladoceran diversity is comparable with many permanent systems in the region and other temporary pool systems around the world, and underlines the conservation value of these peatland systems (bofedales) for the aquatic biodiversity of South America.

Para mas informacion aproximarse por : Unidad de Limnología y Recursos Acuáticos (ULRA) en la facultade de Ciencias y Tecnología


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Título del Articulo: Developmental costs of rapid growth in a damselfly

Autores UMSS: Campero M.,

Autores: De Block M., Stoks R.

Facultad: Ciencias y Tecnología

Centro: Unidad de Limnología y Recursos Acuáticos (ULRA)

Editorial: Blackwell Scientific Publications

Fecha de Publicación: 2008     Numero de Paginas 313-318

Abstract:
1. Developmental costs of rapid growth in terms of increased fluctuating asymmetry are expected to contribute to the widespread occurrence of growth rates below the physiological maximum, but have rarely been demonstrated. Here, these costs are studied for the first time in an invertebrate, the damselfly Lestes viridis, using a rearing experiment where early‐ and late‐hatched larvae of both sexes were reared at decreasing or permanent water levels.2. Late‐hatched animals were more asymmetrical than early‐hatched animals except for males in the drying treatment. Also, females were more asymmetrical than males except in early‐hatched animals in the drying treatment.3. The data presented suggest that in females but not in males treatment groups with higher growth rates have more asymmetrical wings. However, at the individual level no relationship between growth rate and asymmetry was present.4. Possible reasons why the suggested trade‐off between growth and developmental instability was not present at the individual level, and at the group level only in females, are discussed.

Para mas informacion aproximarse por : Unidad de Limnología y Recursos Acuáticos (ULRA) en la facultade de Ciencias y Tecnología


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Título del Articulo: Development of a semi-automated model identification and calibration tool for conceptual modelling of sewer systems

Autores UMSS: Villazon M.F.

Autores: Wolfs V., Willems P.

Facultad: Ciencias y Tecnología

Centro: Laboratorio de Hidráulica

Editorial: International Water Association, London

Fecha de Publicación: 2013     Numero de Paginas 167-175

Abstract:
Applications such as real-time control, uncertainty analysis and optimization require an extensive number of model iterations. Full hydrodynamic sewer models do not suffice for these applications due to the excessive computation time. Simplifications are consequently required. A lumped conceptual modelling approach results in a much faster calculation. The process of identifying and calibrating the conceptual model structure could, however, be time consuming. Moreover, many conceptual models lack accuracy, or do not account for backwater effects. To overcome these problems, a modelling methodology was developed which is suited for semi-automatic calibration. The methodology is tested for the sewer system of the city of Geel in the Grote Nete river basin in Belgium, using both synthetic design storm events and long time series of rainfall input. A MATLAB/Simulink® tool was developed to guide the modeller through the step-wise model construction, reducing significantly the time required for the conceptual modelling process.

Para mas informacion aproximarse por : Laboratorio de Hidráulica en la facultade de Ciencias y Tecnología


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Título del Articulo: Pathogens and fecal indicators in waste stabilization pond systems with direct reuse for irrigation: Fate and transport in water, soil and crops

Autores UMSS: Iriarte M.M., Mercado Guzmán A., Coronado O.,

Autores: Verbyla M.E.,Almanza M., Mihelcic J.R.

Facultad: Ciencias y Tecnología

Centro: Centro de Aguas y Saneamiento Ambiental (CASA)

Editorial: Elsevier B.V.

Fecha de Publicación: 2016     Numero de Paginas 551–552

Abstract:
Wastewater use for irrigation is expanding globally, and information about the fate and transport of pathogens in wastewater systems is needed to complete microbial risk assessments and develop policies to protect public health. The lack of maintenance for wastewater treatment facilities in low-income areas and developing countries results in sludge accumulation and compromised performance over time, creating uncertainty about the contamination of soil and crops. The fate and transport of pathogens and fecal indicators was evaluated in waste stabilization ponds with direct reuse for irrigation, using two systems in Bolivia as case studies. Results were compared with models from the literature that have been recommended for design. The removal of Escherichia coli in both systems was adequately predicted by a previously-published dispersed flow model, despite more than 10 years of sludge accumulation. However, a design equation for helminth egg removal overestimated the observed removal, suggesting that this equation may not be appropriate for systems with accumulated sludge. To assess the contamination of soil and crops, ratios were calculated of the pathogen and fecal indicator concentrations in soil or on crops to their respective concentrations in irrigation water (termed soil-water and crop-water ratios). Ratios were similar within each group of microorganisms but differed between microorganism groups, and were generally below 0.1 mL g− 1 for coliphage, between 1 and 100 mL g− 1 for Giardia and Cryptosporidium, and between 100 and 1000 mL g− 1 for helminth eggs. This information can be used for microbial risk assessments to develop safe water reuse policies in support of the United Nations' 2030 Sustainable Development Agenda.

Sitio web: sciencedirect

Para mas informacion aproximarse por : Centro de Aguas y Saneamiento Ambiental (CASA) en la facultade de Ciencias y Tecnología


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Título del Articulo: Phytoplankton-bacterioplankton interactions in a neotropical floodplain lake (Laguna Bufeos, Bolivia)

Autores UMSS: Rejas D.

Autores: Muylaert K., De Meester L.

Facultad: Ciencias y Tecnología

Centro: Unidad de Limnología y Recursos Acuáticos (ULRA)

Editorial: Springer, Dordrecht

Fecha de Publicación: 2005     Numero de Paginas 91-99

Abstract:
Laguna Bufeos is a floodplain lake of the river Ichilo, a tributary of the Amazon basin situated in Bolivia. Nutrient addition assays involving whole water (<200 μm) as well as fractionated water (<0.8 μm) treatments were carried out in incubation tubes to test whether bacterial growth is limited by the availability of inorganic nutrients and to test whether bacteria are able to utilize inorganic nutrients directly or are stimulated by inorganic nutrients through increased production of phytoplankton. The assays were carried out during two extreme hydrological conditions, the high-water and the low-water period. During the high-water period experiment, neither N or P limited bacterial growth rates. During the low-water period, bacterial growth was P limited. Bacterial growth was stimulated in the fractionated as well as in the whole water treatments, indicating that bacterial growth was directly stimulated by P. Bacterial growth corrected for grazing losses (determined by means of dilution experiments) was significantly higher in the fractionated water containing only bacteria when compared to the whole water containing also grazers and phytoplankton. This suggests that bacterial growth was suppressed by competition with phytoplankton rather than stimulated through the production of dissolved organic matter by phytoplankton.

Para mas informacion aproximarse por : Unidad de Limnología y Recursos Acuáticos (ULRA) en la facultade de Ciencias y Tecnología


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Título del Articulo: Regeneration patterns of Polylepis subtusalbida growing with the exotic trees Pinus radiata and Eucalyptus globulus at Parque Nacional Tunari, Bolivia

Autores UMSS: Gareca E.E., Martinez Y.Y., Aguirre L.F.

Autores: Bustamante R.O.,Siles M.M.

Facultad: Ciencias y Tecnología

Centro: Centro de Biodiversidad y Genética (CBG)

Editorial: Springer

Fecha de Publicación: 2007     Numero de Paginas 253–263

Abstract:
The establishment of exotic trees such as Pinus radiata (radiata pine, Pinaceae) and Eucalyptus globulus (eucalyptus, Myrtaceae) in fragments of native vegetation at the Parque Nacional Tunari (PNT, Cochabamba-Bolivia), can affect the regeneration of the native species that live there. Polylepis subtusalbida’s (kewiña, Rosaceae) regeneration was surveyed, evaluating the density of seedlings, their size structure, and growth forms, as well as variables of growth and survival in fragments of pure forests (P. subtusalbida fragments without exotic trees) and mixed fragments (P. subtusalbida fragments with exotic trees). Sixty-four permanent study plots were established in mixed fragments of P. subtusalbida–P. radiata, P. subtusalbida–E. globulus and fragments with only P. subtusalbida, in three locations within the Parque Nacional Tunari. Mixed fragments with both native and exotic trees and native pure fragments did not present differences in seedling density. Differences in density were related to adult native tree densities.

Para mas informacion aproximarse por : Centro de Biodiversidad y Genética (CBG) en la facultade de Ciencias y Tecnología


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Título del Articulo: An Updated Insight into the Sialotranscriptome of Triatoma infestans: Developmental Stage and Geographic Variations

Autores: Schwarz A., Medrano-Mercado N., Schaub G.A., Struchiner C.J., Bargues M.D., Levy M.Z., Ribeiro J.M.C.

Facultad: Ciencias y Tecnología

Centro: Laboratorio de Inmunoparasitología y Chagas- Departamento de Biología

Editorial: Public Library of Science

Fecha de Publicación: 2014     Numero de Paginas

Abstract:
Triatoma infestans is the main vector of Chagas disease in South America. As in all hematophagous arthropods, its saliva contains a complex cocktail that assists blood feeding by preventing platelet aggregation and blood clotting and promoting vasodilation. These salivary components can be immunologically recognized by their vector's hosts and targeted with antibodies that might disrupt blood feeding. These antibodies can be used to detect vector exposure using immunoassays. Antibodies may also contribute to the fast evolution of the salivary cocktail.

Para mas informacion aproximarse por : Laboratorio de Inmunoparasitología y Chagas- Departamento de Biología en la facultade de Ciencias y Tecnología


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Título del Articulo: Integration of a wood pellet burner and a Stirling engine to produce residential heat and power

Autores UMSS: Cardozo E., Alejo L.

Autores: Erlich C., Malmquist A.

Facultad: Ciencias y Tecnología

Editorial: Elsevier, Kidlington

Fecha de Publicación: 2014     Numero de Paginas 671-680

Abstract:
The integration a Stirling engine with a pellet burner is a promising alternative to produce heat and power for residential use. In this context, this study is focused on the experimental evaluation of the integration of a 20 kWth wood pellet burner and a 1 kWe Stirling engine. The thermal power not absorbed by the engine is used to produce hot water. The evaluation highlights the effects of pellet type, combustion chamber length and cycling operation on the Stirling engine temperatures and thermal power absorbed. The results show that the position of the Stirling engine is highly relevant in order to utilize as much as possible of the radiative heat from the burner. Within this study, only a 5 cm distance change between the Stirling engine and the pellet burner could result in an increase of almost 100 °C in the hot side of the engine.

Sitio web: sciencedirect

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Título del Articulo: Development and validation of a thermodynamic model for the performance analysis of a gamma Stirling engine prototype

Autores UMSS: Araoz J.A., Cardozo E., Alejo L.

Autores: Salomon M., Fransson T.H.

Facultad: Ciencias y Tecnología

Editorial: Elsevier Science

Fecha de Publicación: 2015     Numero de Paginas 16-30

Abstract:
This work presents the development and validation of a numerical model that represents the performance of a gamma Stirling engine prototype. The model follows a modular approach considering ideal adiabatic working spaces; limited internal and external heat transfer through the heat exchangers; and mechanical and thermal losses during the cycle. In addition, it includes the calculation of the mechanical efficiency taking into account the crank mechanism effectiveness and the forced work during the cycle. Consequently, the model aims to predict the work that can be effectively taken from the shaft. The model was compared with experimental data obtained in an experimental rig built for the engine prototype. The results showed an acceptable degree of accuracy when comparing with the experimental data, with errors ranging from +/- 1% to +/- 8% for the temperature in the heater side, less than +/- 1% error for the cooler temperatures, and +/- 1 to +/- 8% for the brake power calculations. Therefore, the model was probed adequate for study of the prototype performance. In addition, the results of the simulation reflected the limited performance obtained during the prototype experiments, and a first analysis of the results attributed this to the forced work during the cycle. The implemented model is the basis for a subsequent parametric analysis that will complement the results presented.

Sitio web: sciencedirect

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Título del Articulo: Diversity and zoogeography of Rotifera (Monogononta) in a flood plain lake of the Ichilo River, Bolivia, with notes on little-known species

Autores UMSS: Ferrufino N.L.

Autores: Segers H., De Meester L.

Facultad: Ciencia y Tecnología

Centro: Unidad de Limnología y Recursos Acuáticos (ULRA)

Editorial: Wiley-VCH, Berlin

Fecha de Publicación: 1998     Numero de Paginas 439-448

Abstract:
Results are presented on a study of the momentary species diversity of Rotifera in “Laguna Bufeos”, a flood plain lake of the Ichilo River in Bolivia. A total of 104 morphospecies of monogonont Rotifera were identified from three samples collected on June 1, 1997. The species record includes 11% Neotropical endemics, 5% pantropical and 13% tropicopolitan taxa. Four taxa, viz. Aspelta lestes Harring and Myers, 1928; Colurella denticauda Carlin, 1939; Ptygura intermedia (Davis, 1867) and Trichocerca kostei Segers, 1993 are newly recorded from South America, 67 morphospecies are new to the Bolivian fauna. Notes are added on some selected taxa. Brachionus amazonica Koste and Robertson, 1983 (stat. nov.) is elevated to morphospecies rank.

Para mas informacion aproximarse por : Unidad de Limnología y Recursos Acuáticos (ULRA) en la facultade de Ciencia y Tecnología


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Título del Articulo: Production of a lipolytic enzyme originating from Bacillus halodurans LBB2 in the methylotrophic yeast Pichia pastoris

Autores UMSS: Vargas V.A.

Autores: Ramchuran S.O., Vargas V.A., Hatti-Kaul R., Karlsson E.N.

Facultad: Ciencias y Tecnología

Centro: Centro de Biotecnología(CBT)

Editorial: Springer, Berlin

Fecha de Publicación: 2006     Numero de Paginas 463-472

Abstract:
A gene encoding a lipolytic enzyme amplified from the alkaliphilic bacterium Bacillus halodurans LBB2 was cloned into the pPICZaB vector and integrated into the genome of the protease deficient yeast strain Pichia pastoris SMD1168H. This previously undescribed enzyme was produced in active form, and cloning in frame with the Saccharomyces cerevisiae secretion signal (a-factor) enabled extracellular accumulation of correctly processed enzyme, with an apparent molecular mass of 30 kDa. In shake-flask cultivations, very low production levels were obtained, but these were significantly improved by use of a “batch-induced” cultivation technique which allowed a maximum enzyme activity of 14,000 U/l using p-nitrophenyl butyrate (C-4) as a substrate and a final extracellular lipolytic enzyme concentration of approximately 0.2 g/l. Partial characterization of the produced enzyme (at pH 9) revealed a preference for the short-chain ester (C-4) and significant but lower activity towards medium (C5-C6) and long (C16 and C18) fatty acid chain-length esters. In addition, the enzyme exhibited true lipase activity (7,300 U/l) using olive oil as substrate and significant levels of phospholipase activity (6,400 U/l) by use of a phosphatidylcholine substrate, but no lysophospholipase activity was detected using a lysophosphatidylcholine substrate.

Para mas informacion aproximarse por : Centro de Biotecnología(CBT) en la facultade de Ciencias y Tecnología


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Título del Articulo: Breeding behaviour and distribution of the tucunaré Cichla aff. monoculus in a clear water river of the Bolivian Amazon

Autores UMSS: Muñoz H.

Autores: Van Damme P.A., Duponchelle F.

Facultad: Ciencias y Tecnología

Centro: Unidad de Limnología y Recursos Acuáticos (ULRA)

Editorial: Blackwell, Oxford

Fecha de Publicación: 2006     Numero de Paginas 1018-1030

Abstract:
The breeding behaviour and distribution of tucunaréCichla aff. monoculus were studied for 2 months during the breeding season in the Paraguá River, Bolivia. Tucunaré were more abundant in the old remnant channels and isolated lakes than in the main river channel. The breeding period was delayed for c. 1 month in the river compared to the old remnant channels and lakes. The batch fecundity ranged between 3712 and 10 355 for females weighing 460 and 1380 g, respectively. Gonad analysis of egg‐guarding or juvenile‐guarding females showed that a female was able to spawn more than once during the breeding season. Nests were significantly larger and deeper in the isolated lakes than in the old remnant channels and in the main river. The maximum depth of the nests also differed significantly, being deeper in the isolated lakes than in the river channel and in the old remnant channels.

Para mas informacion aproximarse por : Unidad de Limnología y Recursos Acuáticos (ULRA) en la facultade de Ciencias y Tecnología



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