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Registro: 16    (1 / 16)

 

Título del Articulo: Halomonas boliviensis sp. nov., an alkalitolerant, moderate halophile isolated from soil around a Bolivian hypersaline lake

Autores UMSS: Quillaguamán J

Autores: Quillaguamán J. - Hatti-Kaul R. - Mattiasson B. - Alvarez M.T. - Delgado^bO.

Facultad: Tecnologia

Centro: Centro de Biotecnologia (CBT)

Editorial: Microbiology Society

Fecha de Publicación: 2004     Numero de Paginas 721-725

Sitio web: Microbiology Society

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  2 / 16
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Registro: 15    (2 / 16)


 

Título del Articulo: A comparison of traditional healers' medicinal plant knowledge in the Bolivian Andes and Amazon

Autores UMSS: Arrazola S

Autores: Vandebroek I., Van Damme P., Van Puyvelde L., Arrazola S., De Kimpe N.

Facultad: Tecnologia

Centro: Centro de Biotecnologia (CBT)

Editorial: El Sevier

Fecha de Publicación: 2004     Numero de Paginas 837-849

Abstract:
Medicinal plant knowledge of two groups of traditional healers was thoroughly studied during a 2-year ethnobotanical survey in the Bolivian Andes (Quechua farmers from Apillapampa) and Amazon rainforest (Yuracaré-Trinitario slash-and-burn cultivators from Isiboro-Sécure National Park), respectively. Both areas represent ecologically and culturally diverse zones, differing in floristic diversity, physical accessibility to health care and degree of modernization, the latter evidenced by presence or intensity in use of modern services such as electricity, water distribution, and materials for house construction. It is generally believed that indigenous people have an impressive knowledge of useful plant species and that this knowledge reflects the plant wealth of their living environment. However, the present study shows that healers’ knowledge of collected medicinal plants (expressed as percentage of plants known by name and use by the majority of healers) is higher in the Andean area characterised by a long history of anthropogenic activity, than in the biodiversity-rich rainforest (protected since 1965).

Sitio web: sciencedirect

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  3 / 16
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Registro: 14    (3 / 16)


 

Título del Articulo: Poly(β-hydroxybutyrate) production by a moderate halophile, Halomonas boliviensis LC1

Autores UMSS: Quillaguamán J

Autores: Quillaguamán J., Delgado O., Mattiasson B., Hatti-Kaul R.

Facultad: Tecnologia

Centro: Centro de Biotecnologia (CBT)

Editorial: El Sevier

Fecha de Publicación: 2006     Numero de Paginas 148-154

Abstract:
The moderate halophile Halomonas boliviensis, isolated from a Bolivian saline soil sample, was able to accumulate poly(β-hydroxybutyrate) (PHB) when grown under conditions of nutrient limitation and excess carbon source. The concentration of sodium chloride in the medium influenced the cell-growth, -size, and rate of PHB accumulation. Cultivation in shake flasks led to a PHB accumulation of about 54 wt.% with respect to cell dry weight at 4.5% (w/v) NaCl in a medium with butyric acid and sodium acetate as carbon sources.

Sitio web: sciencedirect

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  4 / 16
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Registro: 13    (4 / 16)


 

Título del Articulo: Clean synthesis of biolubricants for low temperature applications using heterogeneous catalysts

Autores: AKerman C.O., Gaber Y., Ghani N.A., Lämsä M., Hatti-Kaul R.

Facultad: Tecnologia

Centro: Centro de Biotecnologia (CBT)

Editorial: El Sevier

Fecha de Publicación: 2011     Numero de Paginas 263-269

Abstract:
Biolubricants derived from vegetable oils are environmentally compatible products due to their low toxicity and good biodegradability. Synthetic esters based on polyols and fatty acids possess suitable properties for lubricant applications, even at extreme temperatures. In this work, synthesis of esters from trimethylolpropane (TMP) and carboxylic acids from C5 to C18 has been studied and compared using different heterogeneous catalysts (silica–sulphuric acid, Amberlyst-15, and immobilised lipase B from Candida antarctica). Silica–sulphuric acid was found to be the most efficient catalyst followed by Amberlyst-15, especially when using short chain carboxylic acids.

Sitio web: sciencedirect

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  5 / 16
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Registro: 12    (5 / 16)


 

Título del Articulo: Chemo-enzymatic epoxidation of oleic acid and methyl oleate in solvent-free medium

Autores: Orellana-Coca C., Törnvall U., Adlercreutz D., Mattiasson B., Hatti-Kaul R.

Facultad: Tecnologia

Centro: Centro de Biotecnologia (CBT)

Editorial: Taylor Francis Online

Fecha de Publicación: 2005     Numero de Paginas 431-437

Abstract:
Chemo-enzymatic epoxidation of oleic acid (OA) and its methyl ester has been performed using hydrogen peroxide and immobilized lipase from Candida antarctica (Novozym® 435). The purpose of the study was to characterize the reaction under solvent-free conditions. The reaction temperature had a significant impact on epoxidation of OA. At lower temperatures, the substrate conversion was hindered by the formation of solid epoxystearic acid product. Nearly 90% conversion of OA to the epoxide product was obtained after 6 h at 50°C. Longer reaction times at 40°C and above resulted in by-product formation and eventually lowered the product yield.

Sitio web: tandfonline

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  6 / 16
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Registro: 11    (6 / 16)

 

Título del Articulo: Poly(3-hydroxybutyrate) production by Halomonas boliviensis in fed-batch culture

Autores UMSS: Quillaguamán J., Guzmán H., Guzmán D.

Autores: Doan-Van T., Martín J., Everest A., Hatti-Kaul R.

Facultad: Tecnología

Centro: Centro de Biotecnología (CBT)

Editorial: Spring Nature

Fecha de Publicación: 2008     Numero de Paginas 227-232

Abstract:
High poly(3-hydroxybutyrate) (PHB) content and volumetric productivity were achieved by fed-batch culture of Halomonas boliviensis using a defined medium. Initial shake flask cultivations in a minimal medium revealed that the growth of H. boliviensis was supported only when the medium was supplemented with aspartic acid, glycine, or glutamine. Addition of 0.1% (w/v) glutamine in the medium resulted in the highest cell dry weight (CDW; 3.9 g l−1). Glutamine was replaced by the less expensive monosodium glutamate (MSG) in the medium without any notable change in the final cell density. Effect of initial concentrations of NH4Cl and K2HPO4 on cell growth and PHB accumulation by H. boliviensis was then analyzed using a fed-batch fermentation system. The best conditions for PHB production by H. boliviensis were attained using 0.4% (w/v) NH4Cl and 0.22% (w/v) K2HPO4 and adding MSG intermittently to the fermentor. Poly(3-hydroxybutyrate) content and CDW reached 90 wt.% and 23 g l−1, respectively, after 18 h of cultivation. In order to increase CDW and PHB content, MSG, NH4Cl, and K2HPO4 were initially fed to the fermentor to maintain their concentrations at 2%, 0.4%, and 0.22% (w/v), respectively, and subsequently their feed was suppressed. This resulted in a CDW of 44 g l−1, PHB content of 81 wt.%, and PHB volumetric productivity of 1.1 g l−1 h−1.

Sitio web: link.springer

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  7 / 16
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Registro: 10    (7 / 16)


 

Título del Articulo: Lipase mediated simultaneous esterification and epoxidation of oleic acid for the production of alkylepoxystearates

Autores UMSS: Cecilia Orellana-Coca

Autores: Billakanti J.M., Mattiasson B., Hatti-Kaul R.

Facultad: Tecnología

Centro: Centro de Biotecnología (CBT)

Editorial: El Sevier

Fecha de Publicación: 2007     Numero de Paginas 133-137

Abstract:
Epoxy alkylstearates were synthesized by lipase catalysed esterification and perhydrolysis followed by epoxidation of oleic acid in a one-pot process. Immobilized Candida antarctica lipase (Novozym®435) was used as the catalyst. The esterification reaction occurred relatively quickly and was followed by epoxidation of the alkyl ester and the remaining fatty acid. Higher degree of esterification was achieved with n-octanol, n-hexanol and n-butanol as compared to that with ethanol and iso-propanol. The rate and yield of epoxidation was enhanced with iso-propanol but was lowered with the other alcohols. The lipase suffered significant loss in activity during the reaction primarily due to hydrogen peroxide. The presence of alcohols, in particular ethanol, further contributed to the enzyme inactivation. The epoxidation reaction could be improved by step-wise addition of the lipase.

Sitio web: sciencedirect

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  8 / 16
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Registro: 9    (8 / 16)

 

Título del Articulo: Optimizing conditions for poly(β-hydroxybutyrate) production by Halomonas boliviensis LC1 in batch culture with sucrose as carbon source

Autores UMSS: Quillaguamán J

Autores: Muñoz M., Mattiasson B., Hatti-Kaul R.

Facultad: Tecnología

Centro: Centro de Biotecnología (CBT)

Editorial: Springer Link

Fecha de Publicación: 2007     Numero de Paginas 981-986

Abstract:
Halomonas boliviensis LC1 is able to accumulate poly(β-hydroxybutyrate) (PHB) under conditions of excess carbon source and depletion of essential nutrients. This study was aimed at an efficient production of PHB by growing H. boliviensis to high cell concentrations in batch cultures. The effect of ammonium, phosphate, and yeast extract concentrations on cell concentration [cell dry weight (CDW)] and PHB content of H. boliviensis cultured in shake flasks was assayed using a factorial design. High concentrations of these nutrients led to increments in cell growth but reduced the PHB content to some extent. Cultivations of H. boliviensis under controlled conditions in a fermentor using 1.5% (w/v) yeast extract as N source, and intermittent addition of sucrose to provide excess C source, resulted in a polymer accumulation of 44 wt.% and 12 g l−1 CDW after 24 h of cultivation. Batch cultures in a fermentor with initial concentrations of 2.5% (w/v) sucrose and 1.5% (w/v) yeast extract, and with induced oxygen limitation, resulted in an optimum PHB accumulation, PHB concentration and CDW of 54 wt.%, 7.7 g l−1 and 14 g l−1, respectively, after 19 h of cultivation. The addition of casaminoacids in the medium increased the CDW to 14.4 g l−1 in 17 h but reduced the PHB content in the cells to 52 wt.%.

Sitio web: link.springer

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  9 / 16
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Registro: 8    (9 / 16)


 

Título del Articulo: Accounting for detectability improves estimates of species richness in tropical bat surveys

Autores UMSS: Luis F. Aguirre

Autores: Meyer C.F.J., Aguiar L.M.S., Baumgarten J., Clarke F.M., Cosson J.-F., Villegas S.E., Fahr J., Faria D., Furey N., Henry M., Hodgkison R., Jenkins R.K.B., Jung K.G., Kingston T., Kunz T.H., Cristina Macswiney Gonzalez M., Moya I., Patterson B.D., Pons J.-M., Racey P.A., Rex K., Sampaio E.M., Solari S., Stoner K.E., Voigt C.C., von Staden D., Weise C.D., Kalko E.K.V.

Facultad: Tecnología

Centro: entro de Biotecnología (CBT)

Fecha de Publicación: 2011     Numero de Paginas 777-787

Abstract:
Species richness is a state variable of some interest in monitoring programmes but raw species counts are often biased due to imperfect species detectability. Therefore, monitoring programmes should quantify detectability for target taxa to assess whether it varies over temporal or spatial scales. We assessed the potential for tropical bat monitoring programmes to reliably estimate trends in species richness. Using data from 25 bat assemblages from the Old and New World tropics, we estimated detectability for all species in an assemblage (mean proportion of species detected per sampling plot) and for individual species (species-specific detectability). We further assessed how these estimates of detectability were affected by external sources of variation relating to time, space, survey effort and biological traits. The mean proportion of species detected across 96 sampling plots was estimated at 0.76 (range 0.57-1.00) and was significantly greater for phytophagous than for animalivorous species. Species-averaged detectability for phytophagous species was influenced by the number of surveys and season, whereas the number of surveys and sampling methods [ground- or canopy-level mist nets, harp traps and acoustic sampling (AS)] most strongly affected estimates of detectability for animalivorous bats. Species-specific detectability averaged 0.4 and was highly heterogeneous across 232 species, with estimates ranging from 0.03 to 0.84. Species-level detectability was influenced by a range of external factors such as location, season, or sampling method, suggesting that raw species counts may sometimes be strongly biased. Synthesis and applications. Due to generally high species-specific detection probabilities, Neotropical aerial insectivorous bats proved to be well suited for monitoring using AS. However, for species with low detectability, such as most gleaning animalivores or nectarivores, count data obtained in bat monitoring surveys must be corrected for detection bias. Our results indicate that species-averaged detection probabilities will rarely approach 1 unless many surveys are conducted. Consequently, long-term bat monitoring programmes need to adopt an estimation scheme that corrects for variation in detectability when comparing species richness over time and when making regional comparisons. Similar corrections will be needed for other species-rich tropical taxa.

Sitio web: besjournals

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Registro: 7    (10 / 16)

 

Título del Articulo: Lipase-producing microorganisms from a Kenyan alkaline soda lake

Autores UMSS: Vargas V.A

Autores: Delgado O.D., Hatti-Kaul R., Mattiasson B.

Facultad: Tecnología

Centro: Centro de Biotecnología (CBT)

Editorial: Springer, Dordrecht

Fecha de Publicación: 2004     Numero de Paginas 81-86

Abstract:
Lipolytic enzyme production of 150 isolated strains from samples of Lake Bogoria (Kenya) was examined. Among these, fifteen isolates were selected on the basis of their lipolytic activities and subjected to morphological and 16S rRNA gene sequencing analyses for their identification. All the microorganisms have been selected under culture conditions with pH ranges between 7–10 and temperatures of 37–55 °C. Most of them showed optimal growth at 37 °C and tolerated salinity up to 10% (w/v). Ten of the isolates were Gram-negative, nine of which were closely related to the Pseudomonas cluster and one to the Halomonas cluster sharing high similarity profile with Halomonas desiderata. The remaining Gram-positive isolates were closely related to the Bacillus cluster, and were grouped with Bacillus halodurans, Bacillus alcalophilus and Bacillus licheniformis. Four members of the Bacillus cluster and the Halomonas sp. produced lipolytic activity under alkaline conditions, while others did so at neutral pH values.

Sitio web: springer

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Registro: 6    (11 / 16)


 

Título del Articulo: Patterns of roost use by bats in a neotropical savanna: Implications for conservation

Autores UMSS: Aguirre, L.F

Autores: Lens L., Matthysen E.

Facultad: Tecnología

Centro: Centro de Biotecnología(CBT)

Editorial: Elsevier, Oxford

Fecha de Publicación: 2003     Numero de Paginas 435-443

Abstract:
Neotropic bats comprise a highly species-rich group and are considered important regulators of complex ecological processes. Resource partitioning of roosts and food is considered a key element in shaping bat communities. In this paper we examine the roosting patterns of 10 bat species belonging to a neotropical bat community in a Bolivian savanna. Roosts were more common in open woodlands than in forest islands and human settlements. In open woodlands, Tabebuia heptaphylla comprised the largest proportion of roosting trees, whereas in forest islands, Gallesia integrifolia was the most important roosting tree for phyllostomid bats. By comparing active roosts with control holes in Tabebuia heptaphylla, we found that roost site selection by Noctilio albiventris was mainly based on tree diameter and inner width of the cavities. The earlier results are discussed in view of resource partitioning within savanna bat communities and their practical conservation.

Sitio web: sciencedirect

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Registro: 5    (12 / 16)

 

Título del Articulo: A process for the production of ectoine and poly(3-hydroxybutyrate) by Halomonas boliviensis

Autores UMSS: Guzman H., Quillaguamán J.

Autores: Van-Thuoc D., Martín J., Hatti-Kaul R.

Facultad: Ciencia y Tecnología

Centro: Centro de Biotecnología(CBT)

Editorial: Springer, Heidelberg

Fecha de Publicación: 2009     Numero de Paginas 1069–1077

Abstract:
The paper reports a study involving the use of Halomonas boliviensis, a moderate halophile, for co-production of compatible solute ectoine and biopolyester poly(3-hydroxybutyrate) (PHB) in a process comprising two fed-batch cultures. Initial investigations on the growth of the organism in a medium with varying NaCl concentrations showed the highest level of intracellular accumulation of ectoine (0.74 g L−1) at 10–15% (w/v) NaCl, while at 15% (w/v) NaCl, the presence of hydroxyectoine (50 mg L−1) was also noted. On the other hand, the maximum cell dry weight and PHB concentration of 10 and 5.8 g L−1, respectively, were obtained at 5–7.5% (w/v) NaCl. A process comprising two fed-batch cultivations was developed—the first culture aimed at obtaining high cell mass and the second for achieving high yields of ectoine and PHB. In the first fed-batch culture, H. boliviensis was grown in a medium with 4.5% (w/v) NaCl and sufficient levels of monosodium glutamate, NH +4, and PO 3−4. In the second fed-batch culture, the NaCl concentration was increased to 7.5% (w/v) to trigger ectoine synthesis, while nitrogen and phosphorus sources were fed only during the first 3 h and then stopped to favor PHB accumulation. The process resulted in PHB yield of 68.5 wt.% of cell dry weight and volumetric productivity of about 1 g L−1 h−1 and ectoine concentration, content, and volumetric productivity of 4.3 g L−1, 7.2 wt.%, and 2.8 g L−1 day−1, respectively. At salt concentration of 12.5% (w/v) during the second cultivation, the ectoine content was increased to 17 wt.% and productivity to 3.4 g L−1 day−1.

Sitio web: link

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Registro: 4    (13 / 16)


 

Título del Articulo: High productivity of ectoines by Halomonas boliviensis using a combined two-step fed-batch culture and milking process

Autores UMSS: Guzmán H., Quillaguaman J.

Autores: Van-Thuoc D., Hatti-Kaul R.

Facultad: Ciencias y Tecnología

Centro: Centro de Biotecnología(CBT)

Editorial: Elsevier, Amsterdam

Fecha de Publicación: 2010     Numero de Paginas 46-51

Abstract:
A process comprising two-step fed-batch cultivation has been investigated for the production of ectoines using the halophilic bacterium Halomonas boliviensis DSM 15516T. The first cultivation was performed under optimal conditions for cell growth and resulted in cell mass concentration of about 41 g l−1 after 24 h of cultivation. During the second cultivation at higher salt concentration, accumulation of ectoines increased while cell mass decreased with increasing salt concentration. Maximum productivity of total ectoines reached was 10 g l−1 d−1 with ectoine concentration of 6 g l−1 and hydroxyectoine concentration of 8 g l−1 after 9 h of cultivation at 18.5% NaCl, which is among the highest reported so far. H. boliviensis cells were further recycled for the production process after releasing the ectoines. About 75% of the accumulated ectoines were released by subjecting the cells to hypoosmotic shock. On subsequent reincubation in a medium containing higher salt concentration the cells were able to re-synthesize the ectoines resulting in a global productivity of 11.1 g l−1 d−1, and ectoine and hydroxyectoine productivities of 9.1 g l−1 d−1 and 2.0 g l−1 d−1, respectively.

Sitio web: sciencedirect

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Registro: 3    (14 / 16)

 

Título del Articulo: Bacillus bogoriensis sp, nov., a novel alkaliphilic, halotolerant bacterium isolated from a Kenyan soda lake

Autores UMSS: Vargas V.A

Autores: Delgado O.D., Hatti-Kaul R., Mattiasson B.

Facultad: Ciencias y Tecnología

Centro: Centro de Biotecnología(CBT)

Editorial: Society for General Microbiology, Reading

Fecha de Publicación: 2005     Numero de Paginas 899-902

Abstract:
Strain LBB3T isolated from Bogoria soda lake in Kenya is an alkaliphilic, Gram-positive, strictly aerobic, non-motile, spore-forming bacterium. It was identified as a member of the genus Bacillus on the basis of phenotypic and phylogenetic analyses. The organism grows optimally at 37 °C and pH 10. The G+C content of the genomic DNA is 37·5 mol%. 16S rRNA gene sequence analysis showed 95 and 96 % sequence similarity with Bacillus pseudofirmus (DSM 8715T) and Bacillus alcalophilus (DSM 485T), respectively. Furthermore, DNA–DNA hybridization against these two Bacillus species showed 39·0 and 55·5 % similarity, respectively. Based on our observations, strain LBB3T is proposed to represent a novel species of the genus Bacillus, for which the name Bacillus bogoriensis sp. nov. is proposed. The type strain of B. bogoriensis is LBB3T (=ATCC BAA-922T=LMG 22234T).

Sitio web: microbiologyresearch

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Registro: 2    (15 / 16)

 

Título del Articulo: Production of a lipolytic enzyme originating from Bacillus halodurans LBB2 in the methylotrophic yeast Pichia pastoris

Autores UMSS: Vargas V.A.

Autores: Ramchuran S.O., Vargas V.A., Hatti-Kaul R., Karlsson E.N.

Facultad: Ciencias y Tecnología

Centro: Centro de Biotecnología(CBT)

Editorial: Springer, Berlin

Fecha de Publicación: 2006     Numero de Paginas 463-472

Abstract:
A gene encoding a lipolytic enzyme amplified from the alkaliphilic bacterium Bacillus halodurans LBB2 was cloned into the pPICZaB vector and integrated into the genome of the protease deficient yeast strain Pichia pastoris SMD1168H. This previously undescribed enzyme was produced in active form, and cloning in frame with the Saccharomyces cerevisiae secretion signal (a-factor) enabled extracellular accumulation of correctly processed enzyme, with an apparent molecular mass of 30 kDa. In shake-flask cultivations, very low production levels were obtained, but these were significantly improved by use of a “batch-induced” cultivation technique which allowed a maximum enzyme activity of 14,000 U/l using p-nitrophenyl butyrate (C-4) as a substrate and a final extracellular lipolytic enzyme concentration of approximately 0.2 g/l. Partial characterization of the produced enzyme (at pH 9) revealed a preference for the short-chain ester (C-4) and significant but lower activity towards medium (C5-C6) and long (C16 and C18) fatty acid chain-length esters. In addition, the enzyme exhibited true lipase activity (7,300 U/l) using olive oil as substrate and significant levels of phospholipase activity (6,400 U/l) by use of a phosphatidylcholine substrate, but no lysophospholipase activity was detected using a lysophosphatidylcholine substrate.

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Registro: 1    (16 / 16)

 

Título del Articulo: Halomonas andesensis sp. nov., a moderate halophile isolated from the saline lake Laguna Colorada in Bolivia

Autores UMSS: Daniel Guzman, Quillaguamán J.

Autores: Muñoz M., Hatti-Kaul R.

Facultad: Cinecias y Tecnología

Centro: Centro de Biotecnología(CBT)

Fecha de Publicación: 2010     Numero de Paginas 749-753

Abstract:
A moderately halophilic, motile, Gram-negative, rod-shaped bacterium, strain LC6T, was isolated from a water sample of lake Laguna Colorada in the Bolivian Andes. The major cellular fatty acids were C18 : 1 ω7c, iso-C16 : 1 ω7c 2-OH, C16 : 0 and C12 : 0 3-OH. The respiratory ubiquinones found in strain LC6T were Q-9 (97 %) and Q-8 (3 %). Strain LC6T was aerobic, heterotrophic, and able to utilize various carbohydrates and other substrates as carbon source. The G+C content of the genomic DNA of strain LC6T was 52.5 mol%. The organism was able to grow at pH 6.0–11.0 (optimum, pH 7.0–8.0), at 4–45 °C (optimum, 30–35 °C) and in the presence of 0.5–20 % (w/v) NaCl (optimum, 1–3 %, w/v). Based on 16S rRNA gene sequence analysis, strain LC6T was most closely related to Halomonas hydrothermalis DSM 15725T and Halomonas venusta DSM 4743T (98.8 % similarity), followed by Halomonas aquamarina DSM 30161T, Halomonas axialensis DSM 15723T and Halomonas meridiana DSM 5425T (98.4 %). However, levels of DNA–DNA relatedness between strain LC6T and the above type strains were low (<31 %).

Sitio web: microbiologyresearch

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